Sp​ilianakisLab

COURSE BIOL414 - WHEN BIOCHEMISTRY MEETS EPIGENETICS
TYPE OF COURSE Ellective
YEAR 2nd-4th year undergraduate Biology students
SEMESTER Spring
CREDITS 3 (ECTS=5)
DEPARTMENT Biology, University of Crete
LECTURES Biology Auditorium I, Tuesday 16:00-18:00
COURSE MATERIAL In Google Classroom (class code: ​exmrmy4) you can have access to i. textbooks of the course, ii. Powerpoint presentations of the lectures, iii. pdf files of the lecture presentations, iv. previous exams questions & answers, v. Video files of ZOOM lectures, vi. educational videos.
  • Epigenetics
  • D.Allis-T.Jenuwein-D.Reinberg
  • CSHL Press
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  • Handbook of Epigenetics
  • T. Tollefsbol
  • Elsevier
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  • Epigenetics in Biology and Medicine
  • M.Esteller
  • CRC Press Taylor & Francis Group
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  • Epigenetics 
  • Lyle Armstrong
  • University of Newcastle uponTyne, UK
  • Garland Science
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Lectures content and download

INFO 2021

Download: INFO pptx (INFO pdf)

LECTURE 1

- Introduction to Epigenetics
- The history of Epigenetics in CSHL Symposia


LECTURE 2

- Model organisms for the study of Epigenetics
- Definition of Epigenetics
- The basis of the transcription process
- DNA packaging and chromatin architecture


LECTURE 3

- Modifying chromatin structure
- Nucleosome remodeling complexes
- Chromatin modification
- DNA methylation


LECTURE 4

Chromatin modifications and their mechanism of Action
- Histones and acetylation regulate transcription
- Acetylation and Deacetylation
- Phosphorylation
- Methylation
- Ubiquitinylation
- Sumoylation
- Open questions in histone modifications
- Histone code


LECTURE 5

RNAi and Heterochromatin Assembly
- Overview of the RNAi pathway   
- Early evidence implicating RNA as an intermediate in TGS   
- RNAi and heterochromatin assembly in S.pombe   
- Conservation of RNAi-mediated chromatin modifications in animals


LECTURE 6

- Analysis of Gene-specific DNA Methylation
- Methods for Assessing Genome-wide DNA Methylation
- Epigenetic Technology
- Chromatin Modifications Distinguish Genomic Features and Physical Organization of the Nucleus
- Assessing Epigenetic Information.


LECTURE 7

 - Metabolic regulation of DNA methylation in mammals
- Dietary and metabolic compounds affecting chromatin dynamics/remodeling


Download: Lecture 7 pptx (Lecture 7 pdf)